Vintage venoms: Proteomic and pharmacological stability of snake venoms stored for up to eight decades

C Jesupret; K Baumann; TNW Jackson; SA Ali; DC Yang; L Greisman; L Kern; J Steuten; M Jouiaei; NR Casewell; EAB Undheim; I Koludarov; J Debono; DHW Low; S Rossi; N Panagides; K Winter; V Ignjatovic; R Summerhayes; A Jones; A Nouwens; N Dunstan; WC Hodgson; KD Winkel; P Monagle; BG Fry

Journal article

Vintage venoms: Proteomic and pharmacological stability of snake venoms stored for up to eight decades

C Jesupret, K Baumann, TNW Jackson, SA Ali, DC Yang, L Greisman, L Kern, J Steuten, M Jouiaei, NR Casewell, EAB Undheim, I Koludarov, J Debono, DHW Low, S Rossi, N Panagides, K Winter, V Ignjatovic, R Summerhayes, A Jones Show all

Journal of Proteomics | Published : 2014

DOI: 10.1016/j.jprot.2014.01.004

Abstract

For over a century, venom samples from wild snakes have been collected and stored around the world. However, the quality of storage conditions for "vintage" venoms has rarely been assessed. The goal of this study was to determine whether such historical venom samples are still biochemically and pharmacologically viable for research purposes, or if new sample efforts are needed. In total, 52 samples spanning 5 genera and 13 species with regional variants of some species (e.g., 14 different populations of Notechis scutatus) were analysed by a combined proteomic and pharmacological approach to determine protein structural stability and bioactivity. When venoms were not exposed to air during sto..

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University of Melbourne Researchers

Timothy Jackson Author

Vera Ignjatovic Author

Kenneth Winkel Author

Paul Monagle Author

Grants

Awarded by Alabama Commission on Higher Education

Funding Acknowledgements

BGF was funded by an Australian Research Council Future Fellowship and by the University of Queensland. SAA was the recipient of postdoctoral fellowship (PDRF Phase II Batch-V) from Higher Education Commission (HEC Islamabad) Pakistan. TNWJ was funded by an Australian Postgraduate Award. EABU acknowledges funding from the University of Queensland (International Postgraduate Research Scholarship, UQ Centennial Scholarship, and UQ Advantage Top-Up Scholarship) and the Norwegian State Education Loans Fund. NRC acknowledges the support from a Natural Environment Research Council (NERC, UK) fellowship and a UQ Travel Award for International Collaborative Research. KDW is supported by a direct grant from the Australian Government through the National Health and Medical Research Council. LG was supported by a Fulbright Scholarship. Fig. 1 photo is by Ms Lee McRae, University of Melbourne. LG was supported by a Fulbright Scholarship.