Journal article
The novel zinc finger protein dASCIZ regulates mitosis in Drosophila via an essential role in dynein light-chain expression
O Zaytseva, N Tenis, N Mitchell, SI Kanno, A Yasui, J Heierhorst, LM Quinn
Genetics | GENETICS SOCIETY AMERICA | Published : 2014
Abstract
The essential zinc finger protein ASCIZ (also known as ATMIN, ZNF822) plays critical roles during lung organogenesis and B cell development in mice, where it regulates the expression of dynein light chain (DYNLL1/LC8), but its functions in other species including invertebrates are largely unknown. Here we report the identification of the Drosophila ortholog of ASCIZ (dASCIZ) and show that loss of dASCIZ function leads to pronounced mitotic delays with centrosome and spindle positioning defects during development, reminiscent of impaired dynein motor functions. Interestingly, similar mitotic and developmental defects were observed upon knockdown of the DYNLL/LC8-type dynein light chain Cutup ..
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Awarded by Japan Society for the Promotion of Science
Funding Acknowledgements
We thank Sabine Jurado and Tony Tiganis for reading the manuscript and Eric Baehrecke for the UAS-Ctp strains. We are grateful to the Bloomington and Vienna Drosophila RNAi Center (VDRC) stock centers for Drosophila strains and to the Developmental Studies Hybridoma Bank (DSHB) for antibodies. Funded by Project Grants and a Senior Research Fellowship from the National Health and Medical Research Council of Australia (ID1009763, ID1025125, and ID1022469) and in part by the Victorian State Governments Operational Infrastructure Support Program to J.H.