Characterization of a Novel Tumorigenic Esophageal Adenocarcinoma Cell Line: OANC1
Nicholas J Clemons, Hongdo Do, Christina Fennell, Siddhartha Deb, Andrew Fellowes, Alexander Dobrovic, Wayne A Phillips
Digestive Diseases and Sciences | SPRINGER | Published : 2014
BACKGROUND: Esophageal adenocarcinoma (EAC) has a very high case fatality rate and is one of the fastest rising cancers worldwide. At the same time, research into EAC has been hampered by a relative lack of pre-clinical models, including representative cell lines. AIM: The purpose of this study was to establish and characterize a new EAC cell line. METHODS: Tumor cells were isolated from EAC tissue by enzymatic digestion. Origin of the cell line was confirmed by microsatellite based genotyping. A panel of cancer-related genes was screened for mutations by targeted deep sequencing, Sanger sequencing and high resolution melting.CDKN2A promoter methylation was assessed by methylation specific h..View full abstract
The authors would like to thank the Peter MacCallum Cancer Centre Department of Pathology for performing immunohistochemistry, FISH and sequencing, the Histology Core in the Research Division for preparation of xenograft tissue blocks, and Dr. James Eshleman for the provision of JH-EsoAd1 cells. The patient history and fresh and FFPE tumour tissue used in this project was provided by the Victorian Cancer Biobank with appropriate ethics approval. The Victorian Cancer Biobank is supported by the Victorian Government, Australia. NJC and WAP are supported by grants from the National Health and Medical Research Council, Australia. This work was supported by grants from the National Health and Medical Research Council, Australia.