Journal article
The effect of paraformaldehyde fixation and sucrose cryoprotection on metal concentration in murine neurological tissue
DJ Hare, JL George, L Bray, I Volitakis, A Vais, TM Ryan, RA Cherny, AI Bush, CL Masters, PA Adlard, PA Doble, DI Finkelstein
Journal of Analytical Atomic Spectrometry | ROYAL SOC CHEMISTRY | Published : 2014
DOI: 10.1039/c3ja50281c
Abstract
Aldehyde preservation of tissue and cryoprotection by sugars and polyols has traditionally been used in histology to preserve structural integrity of samples. The effect of paraformaldehyde (PFA) fixation and sucrose cryoprotection on murine brain metals was studied by examining the changes in total brain metal levels throughout a complete fixation/cryoprotection protocol. Blank-corrected metal levels in 4% PFA and two changes of 30% sucrose solutions following brain immersion were determined by ICP-MS. Fixed and cryoprotected tissue was lyophilised, digested in HNO3/H2O2 and analysed using the same ICP-MS protocol. The sum of metal loss or gain in each solution and total digest was used to ..
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Funding Acknowledgements
This work was supported by funds from the National Health and Medical Research Council and the Australian Research Council. We thank Agilent Technologies and New Wave Research for support through the Australian Research Council Linkage Projects grant scheme (LP120200081).