Journal article

A dual-targeted aminoacyl-tRNA synthetase in Plasmodium falciparum charges cytosolic and apicoplast tRNA(Cys)

James S Pham, Reiko Sakaguchi, Lee M Yeoh, Nilushi S De Silva, Geoffrey I McFadden, Ya-Ming Hou, Stuart A Ralph

BIOCHEMICAL JOURNAL | PORTLAND PRESS LTD | Published : 2014

Abstract

Plasmodium parasites possess two endosymbiotic organelles: a mitochondrion and a relict plastid called the apicoplast. To accommodate the translational requirements of these organelles in addition to its cytosolic translation apparatus, the parasite must maintain a supply of charged tRNA molecules in each of these compartments. In the present study we investigate how the parasite manages these translational requirements for charged tRNACys with only a single gene for CysRS (cysteinyl-tRNA synthetase). We demonstrate that the single PfCysRS (Plasmodium falciparum CysRS) transcript is alternatively spliced, and, using a combination of endogenous and heterologous tagging experiments in both P. ..

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Grants

Awarded by Australian Research Council


Awarded by Australian NHMRC (National Health and Medical Research Council)


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Funding Acknowledgements

This work was supported by the Australian Research Council [grant numbers FT0990350, DP130103236], the Australian NHMRC (National Health and Medical Research Council) [grant numbers 520700, 628704 and 637406 (to S.A.R.)] and an NHMRC program grant (to G.I.M.). J.S.P. and L.M.Y. are supported by Australian Postgraduate Awards. The research stay of J.S.P. with the Hou laboratory at Thomas Jefferson University, Philadelphia, PA, U.S.A. was supported by a J.D. Smyth Postgraduate Student Travel Award from The Australian Society for Parasitology.