Clonality of smooth muscle and fibroblast cell populations isolated from human fibroid and myometrial tissues
Sarah J Holdsworth-Carson, Marina Zaitseva, Beverley J Vollenhoven, Peter AW Rogers
MOLECULAR HUMAN REPRODUCTION | OXFORD UNIV PRESS | Published : 2014
Uterine fibroids are conventionally defined as clonally derived benign tumours from the proliferation of a single smooth muscle cell (SMC). We have previously identified fibroblast-like cells in fibroids, the presence of which raises the question as to whether all cells within the fibroid have the same clonal origin. The first aim of this study was to develop a fluorescence-activated cell sorting (FACS)-based method to isolate different cell types from human myometrium and fibroid tissues. Secondly, we aimed to use X chromosome inactivation analysis to determine the clonality of cell subpopulations isolated from myometrial and fibroid tissues. Human myometrium and fibroid tissues were collec..View full abstract
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Awarded by National Health and Medical Research Council
This work was supported by National Health and Medical Research Council (606613 to P. A. W. R. and B.J.V.).