Enhanced in vitro CA1 network activity in a sodium channel beta 1(C121W) subunit model of genetic epilepsy
Robert J Hatch, Christopher A Reid, Steven Petrou
EPILEPSIA | WILEY-BLACKWELL | Published : 2014
OBJECTIVE: A NaV β1(C121W) mouse model of human genetic epilepsy has enhanced neuronal excitability and temperature sensitivity attributed to a decreased threshold for action potential firing in the axon initial segment. To investigate the network consequences of this neuronal dysfunction and to establish a genetic disease state model we developed an in vitro assay to investigate CA1 network properties and antiepileptic drug sensitivity. METHODS: CA1 network oscillations were induced by tetanic stimulation and average number of spikes, interspike interval (ISI), duration, and latency were measured in slices from control and NaV β1(C121W) heterozygous mice in the presence and absence of retig..View full abstract
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Awarded by NHMRC
Awarded by ARC
Supported by APA to RJH, NHMRC program grant 400121 to SP, and NMHRC fellowship 1005050 to SP. CAR acknowledges the support of the ARC (FT0990628). The Florey Institute of Neuroscience and Mental Health is supported by Victorian State Government infrastructure funds. Authors thank Megan Oliva for contributions to editing.