Enhanced in vitro CA1 network activity in a sodium channel beta 1(C121W) subunit model of genetic epilepsy

Robert J Hatch; Christopher A Reid; Steven Petrou

Journal article

Enhanced in vitro CA1 network activity in a sodium channel beta 1(C121W) subunit model of genetic epilepsy

Robert J Hatch, Christopher A Reid, Steven Petrou

EPILEPSIA | WILEY-BLACKWELL | Published : 2014

DOI: 10.1111/epi.12568

Abstract

OBJECTIVE: A NaV β1(C121W) mouse model of human genetic epilepsy has enhanced neuronal excitability and temperature sensitivity attributed to a decreased threshold for action potential firing in the axon initial segment. To investigate the network consequences of this neuronal dysfunction and to establish a genetic disease state model we developed an in vitro assay to investigate CA1 network properties and antiepileptic drug sensitivity. METHODS: CA1 network oscillations were induced by tetanic stimulation and average number of spikes, interspike interval (ISI), duration, and latency were measured in slices from control and NaV β1(C121W) heterozygous mice in the presence and absence of retig..

View full abstract

University of Melbourne Researchers

Steven Petrou Author Florey Department of Neuroscience and Mental Health

Rob Hatch Author Florey Department of Neuroscience and Mental Health

Christopher Reid Author Medicine - Austin Health

Related Projects (5)

Epilepsy: Molecular basis and mechanisms in the era of functional genomics

The team comprises of neurologists with a special interest in epilepsy (both adult and child) molecular geneticists, physiologists and brain..

Epilepsy: Molecular basis and mechanisms in the era of functional genomics

The team comprises of neurologists with a special interest in epilepsy (both adult and child) molecular geneticists, physiologists and brain..

How gene mutations cause epilepsy

Variation in our genetic makeup can cause serious brain disorders such as epilepsy. The goal of this research is to determine how variation ..

Epilepsy: Molecular basis and mechanisms in the era of functional genomics

The team comprises of neurologists with a special interest in epilepsy (both adult and child) molecular geneticists, physiologists and brain..

How gene mutations cause epilepsy

Variation in our genetic makeup can cause serious brain disorders such as epilepsy. The goal of this research is to determine how variation ..

Grants

Awarded by NHMRC

Awarded by ARC

Funding Acknowledgements

Supported by APA to RJH, NHMRC program grant 400121 to SP, and NMHRC fellowship 1005050 to SP. CAR acknowledges the support of the ARC (FT0990628). The Florey Institute of Neuroscience and Mental Health is supported by Victorian State Government infrastructure funds. Authors thank Megan Oliva for contributions to editing.