Techniques for Processing Eyes Implanted With a Retinal Prosthesis for Localized Histopathological Analysis
David AX Nayagam, Ceara McGowan, Joel Villalobos, Richard A Williams, Cesar Salinas-LaRosa, Penny McKelvie, Irene Lo, Meri Basa, Justin Tan, Chris E Williams
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | JOURNAL OF VISUALIZED EXPERIMENTS | Published : 2013
With the recent development of retinal prostheses, it is important to develop reliable techniques for assessing the safety of these devices in preclinical studies. However, the standard fixation, preparation, and automated histology procedures are not ideal. Here we describe new procedures for evaluating the health of the retina directly adjacent to an implant. Retinal prostheses feature electrode arrays in contact with eye tissue. Previous methods have not been able to spatially localize the ocular tissue adjacent to individual electrodes within the array. In addition, standard histological processing often results in gross artifactual detachment of the retinal layers when assessing implant..View full abstract
This work was performed at the Bionics Institute and St Vincent's Hospital, Melbourne. Funding was provided by the Ian Potter Foundation, the John T Reid Charitable Trusts, and the Australian Research Council through its Special Research Initiative in Bionic Vision Science and Technology grant to Bionic Vision Australia (BVA). The Bionics Institute acknowledges the support it receives from the Victorian Government through its Operational Infrastructure Support Program.