Journal article
Comparison of the DNA sequences and secondary structure of the mitochondrial 16S rRNA gene of Ixodes kingi, Ixodes sculptus and Ixodes angustus
Clare A Anstead, Neil B Chilton
MOLECULAR AND CELLULAR PROBES | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2014
Abstract
The DNA sequences and predicted secondary structure of domains IV and V of the mitochondrial (mt) 16S rRNA gene were compared for three species of Ixodes: Ixodes kingi, Ixodes sculptus and Ixodes angustus. Each species had a unique set of DNA sequences for the 16S gene. Many of the differences in DNA sequence within and among species occurred in a "hypervariable" region of domain V, and either represented partial or full compensatory base pair changes that maintained the helices within the secondary structure, or nucleotide alterations at unpaired positions that had no effect on the secondary structure. The results of the phylogenetic analyses revealed that I. kingi, I. sculptus and I. angus..
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Funding Acknowledgements
Financial support for this work was provided (to NBC) from the Natural Sciences and Engineering Research Council of Canada and the Canadian Foundation for Innovation. A Margaret McKay scholarship and a University of Saskatchewan Graduate Scholarship provided financial support to CAA. This work was approved by the University of Saskatchewan's Animal Research Ethics Board, and adhered to the Canadian Council on Animal Care guidelines for humane animal use. We thank Karen Gesy for providing the specimens of Nodes ricinus and Wayne Lerch for providing the small mammals from Radisson.