EGFR gene copy number alterations are not a useful screening tool for predicting EGFR mutation status in lung adenocarcinoma
Prudence A Russell, Yong Yu, Hongdo Do, Timothy D Clay, Melissa M Moore, Gavin M Wright, Matthew Conron, Zoe Wainer, Alexander Dobrovic, Sue-Anne McLachlan
PATHOLOGY | ELSEVIER | Published : 2014
We investigated if gene copy number (GCN) alterations of the epidermal growth factor receptor (EGFR), as detected by silver enhanced in situ hybridisation (SISH), could be used to select patients for EGFR mutation testing. Resected lung adenocarcinoma specimens with adequate tumour were identified. EGFR SISH was performed using the Ventana Benchmark Ultra platform. EGFR GCN was classified according to the Colorado Classification System. EGFR mutations were scanned by high resolution melting and confirmed by Sanger sequencing. Thirty-four of 96 tumours were EGFR SISH positive (35%), and 31 of 96 tumours harboured one or more EGFR mutations (32%). Of 31 EGFR-mutant tumours, 18 were EGFR SISH p..View full abstract
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Awarded by National Health and Medical Research Council
Funding for this study was provided by St Vincent's Hospital research endowment fund and a concept development grant from the Australasian Lung Cancer Trials Group. TDC was supported by Australian Postgraduate Award from the University of Melbourne. GMW was supported by National Health and Medical Research Council Scholarship GNT 1038699 and University of Melbourne Medical Postgraduate Committee Gordon-Taylor Scholarship. The authors state that there are no conflicts of interest to disclose.