Journal article

In vivo imaging and tracking of individual nanodiamonds in drosophila melanogaster embryos

David A Simpson, Amelia J Thompson, Mark Kowarsky, Nida F Zeeshan, Michael SJ Barson, Liam T Hall, Yan Yan, Stefan Kaufmann, Brett C Johnson, Takeshi Ohshima, Frank Caruso, Robert E Scholten, Robert B Saint, Michael J Murray, Lloyd CL Hollenberg

BIOMEDICAL OPTICS EXPRESS | OPTICAL SOC AMER | Published : 2014

Abstract

In this work, we incorporate and image individual fluorescent nanodiamonds in the powerful genetic model system Drosophila melanogaster. Fluorescence correlation spectroscopy and wide-field imaging techniques are applied to individual fluorescent nanodiamonds in blastoderm cells during stage 5 of development, up to a depth of 40 µm. The majority of nanodiamonds in the blastoderm cells during cellularization exhibit free diffusion with an average diffusion coefficient of (6 ± 3) × 10(-3) µm(2)/s, (mean ± SD). Driven motion in the blastoderm cells was also observed with an average velocity of 0.13 ± 0.10 µm/s (mean ± SD) µm/s and an average applied force of 0.07 ± 0.05 pN (mean ± SD). Nanodiam..

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Grants

Awarded by Australian Research Council Centre of Excellence for Quantum Computation and Communication Technology


Awarded by Australian Research Council


Awarded by Discovery Early Career Researcher Award


Funding Acknowledgements

The authors would like to acknowledge the Melbourne Materials Institute at the University of Melbourne for the seed funding to undertake this research. This research was supported in part by the Australian Research Council Centre of Excellence for Quantum Computation and Communication Technology (Project number CE110001027). LCLH acknowledges the support of the Australian Research Council Laureate Fellowship Scheme (FL130100119). MM and RS acknowledge support from the University of Melbourne and Australian Research Council Discovery grant (DP120104443). FC was supported by the Australian Research Council under the Australian Laureate Fellowship (FL120100030) and YY by the Discovery Early Career Researcher Award (DE130100488). The authors acknowledge Professor Jorg Wrachtrup for helpful discussions and Philipp Senn for assistance with sonicating the nanodiamond suspensions.