Buffalo milk transcriptomics

Abstract

We have used high throughput sequencing technology to investigate the nucleic acid content of buffalo milk. Buffalo milk contains cells from which messenger RNA can be isolated for transcriptome sequencing and, relatively large amount of extracellular small RNAs including significant amounts of micro-RNA (miRNA). The transcriptome of cells isolated from buffalo milk or colostrum contains all major milk protein transcripts at relatively high levels, indicating that milk cells encompass a significant proportion of mammary epithelial cells, and milk cell transcriptomics provides insight onto the lactating mammary gland transcriptome. Skim milk from both colostrum and milk contain significant co..