Journal article
The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants
A Gadeyne, C Sánchez-Rodríguez, S Vanneste, S Di Rubbo, H Zauber, K Vanneste, J Van Leene, N De Winne, D Eeckhout, G Persiau, E Van De Slijke, B Cannoot, L Vercruysse, JR Mayers, M Adamowski, U Kania, M Ehrlich, A Schweighofer, T Ketelaar, S Maere Show all
Cell | Published : 2014
Abstract
Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is essential for physiology and development, but the identification and organization of the machinery operating this process remains largely obscure. Here, we identified an eight-core-component protein complex, the TPLATE complex, essential for plant growth via its role as major adaptor module for clathrin-mediated endocytosis. This complex consists of evolutionarily unique proteins that associate closely with core endocytic elements. The TPLATE complex is recruited as dynamic foci at the plasma membrane preceding recruitment of adaptor protei..
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Funding Acknowledgements
The authors would like to thank Wim Grunewald (VIB/UGent, Belgium), Niko Geldner (University of Lausanne, Switzerland), Satoshi Naramoto and Masaru Fujimoto (University of Tokyo, Japan), Inhwan Hwang (Pohang University, Korea), Gerd Jurgens (Tubingen University, Germany), and Christian Luschnig (Vienna University, Austria) for sharing materials; Mansour Karimi and Riet De Rycke (VIB/University of Ghent, Belgium) for vector cloning and IEM attempts; and Luis Vidali (Worchester Polytechnic Institute, USA) for constructive discussions. A. G. was indebted to the Agency for Innovation by Science and Technology for a predoctoral fellowship. S. P. and C. S. R were funded through the Max-Planck Gesellschaft. K. V., S. V., J.V.L. and S. M. are fellows of the Research Foundation - Flanders (FWO). S. D. R. was funded by the Marie-Curie Initial Training Network BRAVISSIMO (PITN-GA-2008-215118). J.R.M and S.Y.B were supported by the National Science Foundation (1121998) (S.Y.B.). Part of this work was carried out using the Stevin Supercomputer Infrastructure at Ghent University, funded by Ghent University, the Hercules Foundation, and the Flemish Government-department EWI. We apologize to colleagues whose work could not be cited due to space limitations.