Protein array of Coxiella burnetii probed with Q fever sera
XiLe Wang, XiaoLu Xiong, Stephen Graves, John Stenos, BoHai Wen
SCIENCE CHINA-LIFE SCIENCES | SCIENCE PRESS | Published : 2013
Coxiella burnetii is the etiological agent of Q fever. To identify its major seroreactive proteins, a subgenomic protein array was developed. A total of 101 assumed virulence-associated recombinant proteins of C. burnetii were probed with sera from mice experimentally infected with C. burnetii and sera from Q fever patients. Sixteen proteins were recognized as major seroreactive antigens by the mouse sera. Seven of these 16 proteins reacted positively with at least 45% of Q fever patient sera. Notably, HspB had the highest fluorescence intensity value and positive frequency of all the proteins on the array when probed with both Q fever patient sera and mouse sera. These results suggest that ..View full abstract
Awarded by National Natural Science Foundation of China
Awarded by National Basic Research Program of China
This work was supported by the National Natural Science Foundation of China (31170161) and National Basic Research Program of China (2010CB530200, 2010CB530205).