Journal article

Protein array of Coxiella burnetii probed with Q fever sera

XiLe Wang, XiaoLu Xiong, Stephen Graves, John Stenos, BoHai Wen

SCIENCE CHINA-LIFE SCIENCES | SCIENCE PRESS | Published : 2013

Abstract

Coxiella burnetii is the etiological agent of Q fever. To identify its major seroreactive proteins, a subgenomic protein array was developed. A total of 101 assumed virulence-associated recombinant proteins of C. burnetii were probed with sera from mice experimentally infected with C. burnetii and sera from Q fever patients. Sixteen proteins were recognized as major seroreactive antigens by the mouse sera. Seven of these 16 proteins reacted positively with at least 45% of Q fever patient sera. Notably, HspB had the highest fluorescence intensity value and positive frequency of all the proteins on the array when probed with both Q fever patient sera and mouse sera. These results suggest that ..

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University of Melbourne Researchers