Journal article
Diversity of the 47-kD HtrA nucleic acid and translated amino acid sequences from 17 recent human isolates of Orientia
J Jiang, DH Paris, SD Blacksell, N Aukkanit, PN Newton, R Phetsouvanh, L Izzard, J Stenos, SR Graves, NPJ Day, AL Richards
Vector Borne and Zoonotic Diseases | Published : 2013
Abstract
Orientia tsutsugamushi, the etiologic agent of potentially fatal scrub typhus, is characterized by a high antigenic diversity, which complicates the development of a broadly protective vaccine. Efficacy studies in murine and nonhuman primate models demonstrated the DNA vaccine candidate pKarp47, based upon the O. tsutsugamushi Karp 47-kD HtrA protein gene, to be a successful immunoprophylactic against scrub typhus. To characterize 47-kD HtrA protein diversity among human isolates of Orientia, we sequenced the full open reading frame (ORF) of the 47-kD HtrA gene and analyzed the translated amino acid sequences of 17 patient isolates from Thailand (n=13), Laos (n=2), Australia (n=1), and the U..
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Awarded by U.S. DoD MIDRP work unit
Awarded by Wellcome Trust Clinical Research Training Fellowship
Funding Acknowledgements
This work was supported by the U.S. DoD MIDRP work unit # 6000.RAD1.J.A0310. Specimen collection in Laos was part of the Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit funded by the Wellcome Trust of Great Britain. We thank the Directors of Mahosot Hospital, the staff of the Microbiology Laboratory and Infectious Disease Ward, Mahosot Hospital, and Sayaphet Rattanavong. D. H. P. was supported by a Wellcome Trust Clinical Research Training Fellowship (grant no. 0789900/Z/06/Z).