Journal article
Comparison of the performance of IFA, CFA, and ELISA assays for the serodiagnosis of acute Q fever by quality assessment
T Herremans, BM Hogema, M Nabuurs, M Peeters, M Wegdam-Blans, P Schneeberger, C Nijhuis, DW Notermans, J Galama, A Horrevorts, IHM van Loo, B Vlaminckx, HL Zaaijer, MP Koopmans, H Berkhout, C Socolovschi, D Raoult, J Stenos, W Nicholson, H Bijlmer
Diagnostic Microbiology and Infectious Disease | Published : 2013
Abstract
The indirect immunofluorescence assay (IFA) is considered the reference method for diagnosing Q fever, but serology is also performed by complement fixation assay (CFA) or enzyme-linked immunosorbent assay (ELISA). However, comparability between these assays is not clear, and therefore a quality assessment was performed. A total of 25 serum samples from negative controls, Q fever patients, and a serial diluted high-positive sample were analyzed in 10 Dutch laboratories. Six laboratories performed CFA, 5 performed IFA, and 5 performed ELISAs. Three international reference laboratories from Australia, France, and the USA also participated in this study. Qualitative values between laboratories ..
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