Journal article

A Novel Association Between Resident Tissue Macrophages and Nerves in the Peripheral Stroma of the Murine Cornea

Yashar Seyed-Razavi, Holly R Chinnery, Paul G McMenamin

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | Published : 2014

Abstract

PURPOSE: To characterize the interactions between resident macrophage populations and nerves in naïve and injured corneas of the mouse eye. METHODS: Corneas from wild-type (WT) C57BL/6J, BALB/cJ, and transgenic Cx3cr1-eGFP mice were subjected to a 1-mm central epithelial debridement injury. The eyes were fixed and immunostained as flat mounts with a range of antibodies to identify macrophages, neurons, and Schwann cells. Interactions between nerves and immune cells were analyzed and quantitated using three-dimensional reconstructions of confocal microscopy images. Naïve eyes acted as controls. RESULTS: A distinctive association between resident immune cells and corneal nerves was noted in th..

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