Quantifying subcellular distribution of fluorescent fusion proteins in cells migrating within tissues
Heather J Melichar, Ou Li, Paul Herzmark, Raghav K Padmanabhan, Jane Oliaro, Mandy J Ludford-Menting, Philippe Bousso, Sarah M Russell, Badrinath Roysam, Ellen A Robey
Immunology and Cell Biology | NATURE PUBLISHING GROUP | Published : 2011
Awarded by NIH
Awarded by California Institute
We thank Tanja Schwikert for generating the memCFP transgenic construct, Jenny Ross, Ying Fang Liao, Kevin Zhou and Jeff LeDue for technical assistance, Ena Ladi for contribution of a control two-photon data set and Yousef Al-Kofahi and Arunachalam Narayanaswamy for valuable input in cell segmentation and tracking. We acknowledge the CHPS Imaging Core for microscopy assistance. This work was supported by the NIH (AI064227, AI065537 and AI065831 to ER, and EB005157 to BR), the HFSP (to ER and SR), the NHMRC (to SR and JO) and the California Institute for Regenerative Medicine post-doctoral fellowship (Training Grant Number T1-00007) (to HJM).