Journal article
Essential Developmental, Genomic Stability, and Tumour Suppressor Functions of the Mouse Orthologue of hSSB1/NABP2
W Shi, AL Bain, B Schwer, F Al-Ejeh, C Smith, L Wong, H Chai, MS Miranda, U Ho, M Kawaguchi, Y Miura, JW Finnie, M Wall, J Heierhorst, C Wicking, KJ Spring, FW Alt, KK Khanna
Plos Genetics | PUBLIC LIBRARY SCIENCE | Published : 2013
Abstract
Single-stranded DNA binding proteins (SSBs) regulate multiple DNA transactions, including replication, transcription, and repair. We recently identified SSB1 as a novel protein critical for the initiation of ATM signaling and DNA double-strand break repair by homologous recombination. Here we report that germline Ssb1-/- embryos die at birth from respiratory failure due to severe rib cage malformation and impaired alveolar development, coupled with additional skeletal defects. Unexpectedly, Ssb1-/- fibroblasts did not exhibit defects in Atm signaling or γ-H2ax focus kinetics in response to ionizing radiation (IR), and B-cell specific deletion of Ssb1 did not affect class-switch recombination..
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Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
This work was supported by National Health and Medical Research Council (NHMRC) Project Grants 552472 and 1031327 (KKK), National Institutes of Health Grants 5R01AI077595 (FWA) and 5T32CA009382 (BS), and a Victorian Government's Operational Infrastructure Support Program (JH). KKK is an NHMRC Senior Principal Research Fellow; JH and CW are NHMRC Senior Research Fellows. FWA is an Investigator of the Howard Hughes Medical Institute. ALB is supported by an NHMRC Biomedical Postgraduate Scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.