PGC-1 alpha and Reactive Oxygen Species Regulate Human Embryonic Stem Cell-Derived Cardiomyocyte Function
Matthew J Birket, Simona Casini, Georgios Kosmidis, David A Elliott, Akos A Gerencser, Antonius Baartscheer, Cees Schumacher, Pier G Mastroberardino, Andrew G Elefanty, Ed G Stanley, Christine L Mummery
Stem Cell Reports | CELL PRESS | Published : 2013
Awarded by European Research Council
Awarded by ZonMW Animal Alternatives
Awarded by Netherlands Genomics Initiative
Awarded by Marie Curie IRG
We thank Richard Davis, Stefan Braam, and Dorien Ward for advice and protocols for the spin EB differentiation method, and for discussions about the data. We also thank Arie Verkerk for help with manuscript preparation, and David Hood (York University, Ontario, Canada) for the PGC-1 alpha promoter plasmid. This research was support by grants from the Human Frontiers Research Program (M. B.), European Research Council (ERC 323182 to C. M.), ZonMW Animal Alternatives (114000101 to S. C.), Netherlands Institute of Regenerative Medicine (M. B.), Rembrandt Institute of Cardiovascular Science (C. M. and G. K.), Netherlands Genomics Initiative (NGI/NWO 05040202 to P. M.), and Marie Curie IRG 247918 (to P. M.). The Seahorse Extracellular Flux Analyzer was purchased with the generous contribution of Dorpmans-Wigmans Stichting. C. L. M. is a cofounder and consultant of Pluriomics bv.