The nature and discriminatory value of urinary neutrophil gelatinase-associated lipocalin in critically ill patients at risk of acute kidney injury
Neil J Glassford, Antoine G Schneider, Shengyuan Xu, Glenn M Eastwood, Helen Young, Leah Peck, Per Venge, Rinaldo Bellomo
INTENSIVE CARE MEDICINE | SPRINGER | Published : 2013
BACKGROUND: Different molecular forms of urinary neutrophil gelatinase-associated lipocalin (NGAL) have recently been discovered. We aimed to explore the nature, source and discriminatory value of urinary NGAL in intensive care unit (ICU) patients. METHODS: We simultaneously measured plasma NGAL (pNGAL), urinary NGAL (uNGAL), and estimated monomeric and homodimeric uNGAL contribution using Western blotting-validated enzyme-linked immunosorbent assays [uNGAL(E1) and uNGAL(E2)] and their calculated ratio in 102 patients with the systemic inflammatory response syndrome and oliguria, and/or a creatinine rise of >25 μmol/L. MEASUREMENTS AND MAIN RESULTS: Bland-Altman analysis demonstrated that, d..View full abstract
This study was supported by the Austin Hospital Intensive Care Trust Fund. The ELISA testing was funded by a grant from the Swedish Medical Research Council to Uppsala University.