The influence of various confounding factors on mechanical nociceptive thresholds in the donkey

Abstract

Objective: To evaluate a mechanical nociceptive threshold (MNT) testing device in the donkey, and to investigate the influence of potential confounders on MNTs generated. Study design: Prospective, randomised. Animals: Sixteen castrated male donkeys aged 4-9 years, weighing 105-170 kg. Methods: Mechanical nociceptive thresholds were measured using an actuator with three pins placed on the dorsal aspect of the distal limb, connected to a force meter. The pins (surface area 15 mm2) were extruded onto the limb by pressurising an air-filled syringe, until the MNT force (when foot-lift was observed) or 25 N (cut-off force) was reached. Effect on MNT of presence of a companion donkey, the limb tes..