Journal article
Molecular evidence for the localization of plasmodium falciparum immature gametocytes in bone marrow
R Aguilar, A Magallon-Tejada, AH Achtman, C Moraleda, R Joice, P Cisteró, CSN Li Wai Suen, A Nhabomba, E Macete, I Mueller, M Marti, PL Alonso, C Menéndez, L Schofield, A Mayor
Blood | Published : 2014
Abstract
Plasmodium falciparum immature gametocytes are not observed in peripheral blood. However, gametocyte stages in organs such as bone marrow have never been assessed bymolecular techniques, which are more sensitive than optical microscopy. We quantified P falciparum sexual stages in bone marrow (n = 174) and peripheral blood (n = 70) of Mozambican anemic children by quantitative polymerase chain reaction targeting transcripts specific for early (PF14-0748; PHISTa), intermediate (PF13-0247; Pfs48/45), and mature (PF10-0303; Pfs25) gametocytes. Among children positive for the P falciparum housekeeping gene (PF08-0085; ubiquitin-conjugating enzyme gene) in bone marrow (n = 136) and peripheral bloo..
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Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
This study received financial support from the Agencia de Cooperacion Internacional de Las Illes Balears, the Fundacion Ramon Areces, and the Bill and Melinda Gates Foundation (Malaria Transmission Epidemiology Project). The CISM receives core support from the Spanish Agency for International Cooperation and Development. The Walter and Eliza Hall Institute receives Victorian State Government Operational Infrastructure Support and Australian Government National Health and Medical Research Council (NHMRC) Independent Research Institutes Infrastructure Support Scheme. L. S. received support from an NHMRC Program Grant; I. M. from an NHMRC Senior Research Fellowship; A.M.-T. from the Secretaria Nacional de Ciencia, Tecnologia e Innovacion de la Republica de Panama; M. M. from the National Institutes of Health, National Institute of Allergy and Infectious Diseases (R01A107755801); and A. M. from the Instituto de Salud Carlos III (CP-04/00220).