Journal article
Stretched cell cycle model for proliferating lymphocytes
MR Dowling, A Kan, S Heinzel, JHS Zhou, JM Marchingo, CJ Wellard, JF Markham, PD Hodgkin
Proceedings of the National Academy of Sciences of the United States of America | Published : 2014
Abstract
Stochastic variation in cell cycle time is a consistent feature of otherwise similar cells within a growing population. Classic studies concluded that the bulk of the variation occurs in the G1 phase, and many mathematical models assume a constant time for traversing the S/G2/M phases. By direct observation of transgenic fluorescent fusion proteins that report the onset of S phase, we establish that dividing B and T lymphocytes spend a near-fixed proportion of total division time in S/G2/M phases, and this proportion is correlated between sibling cells. This result is inconsistent with models that assume independent times for consecutive phases. Instead, we propose a stretching model for div..
View full abstractGrants
Funding Acknowledgements
We thank Carel van Gend, Ken Duffy, Tom Weber, and Edwin Hawkins for discussion and comments on the manuscript; Kelly Rogers, Karl Dudfield, Rajib Chakravorty, David Rawlinson, and Alan Zhang for assistance in establishing long-term microscopy and developing image processing routines; Lynn Corcoran for help with methods; and Dave Tarlinton for help with mouse breeding. FUCCI Red and FUCCI Green mice were provided by the Riken BioResource Centre through the National BioResource Project of the Ministry of Education, Culture, Sports, Science and Technology- Japan. This work was supported by the National Health and Medical Research Council via project Grant 1010654, program Grant 1016647, and fellowships to M. R. D. and P. D. H. as well as Independent Research Institutes Infrastructure Support Scheme Grant 361646. We also received support from the Victorian State Government Operational Infrastructure scheme and National ICT, Australia (NICTA). NICTA is funded by the Australian Government as represented by the Department of Broadband, Communications and the Digital Economy and the Australian Research Council through the ICT Centre of Excellence program. J.H.S.Z. and J.M.M. are the recipients of an Australian Postgraduate Award.