Journal article
Class i to III histone deacetylases differentially regulate inflammation-induced matrix metalloproteinase 9 expression in primary amnion cells
M Poljak, R Lim, G Barker, M Lappas
Reproductive Sciences | SPRINGER HEIDELBERG | Published : 2014
Abstract
Matrix metalloproteinase (MMP) 9 plays an important role in the degradation of the extracellular matrix in fetal membranes, and pathological activation of MMP-9 can lead to preterm birth. In nongestational tissues, modulation of histone deacetylases (HDACs) regulates MMP-9 expression. The aim of this study was to determine whether class I to III HDACs regulate MMP-9 expression and activity in primary amnion cells. Class I and II HDAC regulation of MMP-9 was assessed using the general class I and II HDAC inhibitors (HDACi) trichostatin A (TSA) and suberoylanilide hydroxamic acid (SAHA), the class I HDACi MS-275, and the class II HDACi MC1568. Class III HDAC regulation of MMP-9 was assessed us..
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Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
The author(s) disclosed receipt of the following financial support for the research, authorship and/or publication of this article: Associate Professor Martha Lappas was supported by a Career Development Fellowship from the National Health and Medical Research Council (NHMRC; grant no. 1047025). Dr Ratana Lim was, in part, supported by an early career researcher grant from the University of Melbourne. Funding for the iMark microplate spectrophotometer and Chemidoc XRS system was, in part, provided by the Medical Research Foundation for Women and Babies.