Journal article

Development of a novel polyclonal antibody-based immunoassay for the quantitation of non-albumin urinary proteins

WB Haigh, DM Guralski, LM Arrigo, JT Letsinger, M Clarke, RJ MacIsaac, G Jerums, DL Very

The Open Clinical Chemistry Journal | Published : 2013

Abstract

Background: Better diagnostic methods to detect urinary proteinuria or albuminuria, important indicators of diabetic kidney disease, are needed. Here, we describe the development and performance qualification of a new immunoassay for the quantitation of non-albumin urinary proteins of 20 to 90 kilodaltons. Methods: Urinary proteins, purified from pooled, 24-hour, diabetic urines, were immunoabsorbed to remove albumin, electrophoretically characterized, and identified by mass spectrometry. Sheep anti-urinary protein polyclonal antibodies were immunoabsorbed to remove albumin reactivity. Major immunor..

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