Bronchial epithelial cells are rendered insensitive to glucocorticoid transactivation by transforming growth factor-beta 1
Christine R Keenan, Josephine SL Mok, Trudi Harris, Yuxiu Xia, Saad Salem, Alastair G Stewart
RESPIRATORY RESEARCH | BIOMED CENTRAL LTD | Published : 2014
BACKGROUND: We have previously shown that transforming growth factor-beta (TGF-beta) impairs glucocorticoid (GC) function in pulmonary epithelial cell-lines. However, the signalling cascade leading to this impairment is unknown. In the present study, we provide the first evidence that TGF-beta impairs GC action in differentiated primary air-liquid interface (ALI) human bronchial epithelial cells (HBECs). Using the BEAS-2B bronchial epithelial cell line, we also present a systematic examination of the known pathways activated by TGF-beta, in order to ascertain the molecular mechanism through which TGF-beta impairs epithelial GC action. METHODS: GC transactivation was measured using a Glucocor..View full abstract
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Awarded by NHMRC (Australia)
This work was supported by the NHMRC (Australia) project grant # 1059665.