Journal article
An in vivo model for analysis of developmental erythropoiesis and globin gene regulation
B McColl, BR Kao, P Lourthai, K Chan, H Wardan, M Roosjen, O Delagneau, LJ Gearing, ME Blewitt, S Svasti, S Fucharoen, J Vadolas
FASEB Journal | FEDERATION AMER SOC EXP BIOL | Published : 2014
DOI: 10.1096/fj.13-246637
Abstract
Expression of fetal γ-globin in adulthood ameliorates symptoms of β-hemoglobinopathies by compensating for the mutant β-globin. Reactivation of the silenced γ-globin gene is therefore of substantial clinical interest. To study the regulation of γ-globin expression, we created the GG mice, which carry an intact 183-kb human β-globin locus modified to express enhanced green fluorescent protein (eGFP) from the Gγ-globin promoter. GG embryos express eGFP first in the yolk sac blood islands and then in the aorta-gonad mesonephros and the fetal liver, the sites of normal embryonic hematopoiesis. eGFP expression in erythroid cells peaks at E9.5 and then is rapidly silenced (>95%) and maintained at ..
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Funding Acknowledgements
The authors thank Chelsea Dunn for assistance with the preparation of figures. This work was supported by the National Health and Medical Research Council, the Murdoch Childrens Research Institute, the Victorian Government's Operational Infrastructure Support Program, Thalassaemia Australia, the Thalassaemia Society of New South Wales, the Greek Conference, a Research Chair grant from the Thailand National Science and Technology Development Agency (NSTDA), and Mahidol University. P. L. was supported by the Medical Scholars Program, Mahidol University. P. L. was a Ph.D. candidate at Mahidol University; this work was submitted in partial fulfilment of the Ph.D. requirements.