Characterization of the Stability and Bio-functionality of Tethered Proteins on Bioengineered Scaffolds IMPLICATIONS FOR STEM CELL BIOLOGY AND TISSUE REPAIR
Ting-Yi Wang, Kiara AF Bruggeman, Rebecca K Sheean, Bradley J Turner, David R Nisbet, Clare L Parish
JOURNAL OF BIOLOGICAL CHEMISTRY | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2014
Various engineering applications have been utilized to deliver molecules and compounds in both innate and biological settings. In the context of biological applications, the timely delivery of molecules can be critical for cellular and organ function. As such, previous studies have demonstrated the superiority of long-term protein delivery, by way of protein tethering onto bioengineered scaffolds, compared with conventional delivery of soluble protein in vitro and in vivo. Despite such benefits little knowledge exists regarding the stability, release kinetics, longevity, activation of intracellular pathway, and functionality of these proteins over time. By way of example, here we examined th..View full abstract
We thank Francesca Maclean for electrospinning and Dr. Francesca Ercole for assistance with the determination of attached amines. Access to the facilities of the Centre for Advanced Microscopy (CAM) with funding through the Australian Microscopy and Microanalysis Research Facility (AMMRF) is gratefully acknowledged.