Journal article

Characterization of the Stability and Bio-functionality of Tethered Proteins on Bioengineered Scaffolds IMPLICATIONS FOR STEM CELL BIOLOGY AND TISSUE REPAIR

Ting-Yi Wang, Kiara AF Bruggeman, Rebecca K Sheean, Bradley J Turner, David R Nisbet, Clare L Parish

JOURNAL OF BIOLOGICAL CHEMISTRY | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2014

Abstract

Various engineering applications have been utilized to deliver molecules and compounds in both innate and biological settings. In the context of biological applications, the timely delivery of molecules can be critical for cellular and organ function. As such, previous studies have demonstrated the superiority of long-term protein delivery, by way of protein tethering onto bioengineered scaffolds, compared with conventional delivery of soluble protein in vitro and in vivo. Despite such benefits little knowledge exists regarding the stability, release kinetics, longevity, activation of intracellular pathway, and functionality of these proteins over time. By way of example, here we examined th..

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