Journal article
Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: Implications for stem cell biology and tissue repair
TY Wang, KAF Bruggeman, RK Sheean, BJ Turner, DR Nisbet, CL Parish
Journal of Biological Chemistry | Published : 2014
Abstract
Background: Tethering proteins onto bioengineered scaffolds enables longterm delivery, however protein stability, release kinetics, and functionality over time remains unknown. Results: Tethered proteins remain stable and functional for several months, capable of activating intracellular signaling pathways and influencing cell fate. Conclusion: Tethered proteins are stable and functional long-term. Significance: Such knowledge may have implications for promoting tissue repair. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.
Grants
Funding Acknowledgements
We thank Francesca Maclean for electrospinning and Dr. Francesca Ercole for assistance with the determination of attached amines. Access to the facilities of the Centre for Advanced Microscopy (CAM) with funding through the Australian Microscopy and Microanalysis Research Facility (AMMRF) is gratefully acknowledged.