Journal article
Purification and characterization of a novel restricted antigen expressed by normal and transformed human colonic epithelium
B Catimel, G Ritter, S Welt, LJ Old, L Cohen, MA Nerrie, SJ White, JK Heath, B Demediuk, T Domagala, FT Lee, AM Scott, GF Tu, H Ji, RL Moritz, RJ Simpson, AW Burgess, EC Nice
Journal of Biological Chemistry | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 1996
Abstract
A cell surface antigen that is expressed by normal and 95% of transformed colonic epithelium and is recognized by the monoclonal antibody A33 (Welt, S., Divgi, C. R., Real, F. X., Yeh, S. D., Garin-Chesa, P., Finstad, C. L., Sakamoto, J., Cohen, A., Sigurdson, E. R., Kemeny, N., Carswell, E. A., Oettgen, H. F., and Old, L. J. (1990) J. Clin. Oncol. 8, 1894-1906) has been purified to homogeneity from the human colonic carcinoma cell line LIM1215. The A33 protein was purified from Triton X-114 extracts of LIM1215 cells under nondenaturing conditions. These extracts were applied sequentially to Green-Sepharose HE-4BD, Mono-Q HR 10/10, Superose 12 HR 10/30, and micropreparative Brownlee Aquapore..
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