Journal article
The human A33 antigen is a transmembrane glycoprotein and a novel member of the immunoglobulin superfamily
JK Heath, SJ White, CN Johnstone, B Catimel, RJ Simpson, RL Moritz, GF Tu, H Ji, RH Whitehead, LC Groenen, AM Scott, G Ritter, L Cohen, S Welt, LJ Old, EC Nice, AW Burgess
Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD SCIENCES | Published : 1997
Abstract
The mAb A33 detects a membrane antigen that is expressed in normal human colonic and small bowel epithelium and >95% of human colon cancers. It is absent from most other human tissues and tumor types. The murine A33 mAb has been shown to target colon cancer in clinical trials, and the therapeutic potential of a humanized antibody is currently being evaluated. Using detergent extracts of the human colon carcinoma cell lines LIM1215 and SW1222, in which the antigen is highly expressed, the molecule was purified, yielding a 43-kDa protein. The N-terminal sequence was determined and further internal peptide sequence obtained following enzymatic cleavage. Degenerate primers were used in PCRs to p..
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