CPAP interacts with 14-3-3 in a cell cycle-dependent manner

Abstract

We have previously reported that CPAP (Centrosomal Protein 4.1-Associated Protein) carries a novel microtubule-destabilizing motif that may regulate microtubule dynamics at the centrosome. In this study, we searched for conserved sequence motifs in CPAP and identified two classical 14-3-3 binding sites. The interaction between CPAP and 14-3-3 was demonstrated by both yeast two-hybrid and co-immunoprecipitation experiments. Further analyses revealed that the 14-3-3 binding motif is located within the C-terminal domain of CPAP. Alkaline phosphatase treatment disrupted CPAP binding to 14-3-3, suggesting that phosphorylation is required for the interaction. Mutation of serine 1109 to alanine (S1..