Journal article
Myeloid Dendritic Cells Induce HIV-1 Latency in Non-proliferating CD4 T Cells
VA Evans, N Kumar, A Filali, FA Procopio, O Yegorov, JP Goulet, S Saleh, EK Haddad, C da Fonseca Pereira, PC Ellenberg, RP Sekaly, PU Cameron, SR Lewin
Plos Pathogens | Published : 2013
Abstract
Latently infected resting CD4+ T cells are a major barrier to HIV cure. Understanding how latency is established, maintained and reversed is critical to identifying novel strategies to eliminate latently infected cells. We demonstrate here that co-culture of resting CD4+ T cells and syngeneic myeloid dendritic cells (mDC) can dramatically increase the frequency of HIV DNA integration and latent HIV infection in non-proliferating memory, but not naïve, CD4+ T cells. Latency was eliminated when cell-to-cell contact was prevented in the mDC-T cell co-cultures and reduced when clustering was minimised in the mDC-T cell co-cultures. Supernatants from infected mDC-T cell co-cultures did not facili..
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Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
The authors acknowledge the following funding bodies: American Foundation for AIDS Research (amfAR; 108023; 108237-51-RGRL), www.amfar.org; the National Health and Medical Research Council (NHMRC) of Australia (APP1041795), www.nhmrc.gov.au; and the National Institutes of Health Delaney AIDS Research Enterprise (DARE) to find a cure collaboratory (U19 AI096109), www.delaneycare.org. The authors gratefully acknowledge the contribution to this work of the Victorian Operational Infrastructure Support Program received by the Burnet Institute. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.