Journal article

HIV-specific antibody-dependent phagocytosis matures during HIV infection

Fernanda Ana-Sosa-Batiz, Angus PR Johnston, Haiyin Liu, Robert J Center, Supachai Rerks-Ngarm, Punnee Pitisuttithum, Sorachai Nitayaphan, Jaranit Kaewkungwal, Jerome H Kim, Nelson L Michael, Anthony D Kelleher, Ivan Stratov, Stephen J Kent, Marit Kramski

IMMUNOLOGY AND CELL BIOLOGY | WILEY | Published : 2014

Abstract

Antibody-dependent phagocytosis (ADP) is a potentially important immune mechanism to clear HIV. How HIV-specific ADP responses mature during HIV infection or in response to vaccinations administered, including the partially successful RV144 HIV vaccine, is not known. We established a modified ADP assay to measure internalisation of HIV antibody (Ab)-opsonised targets using a specific hybridisation internalisation probe. Labelled beads were coated with both biotinylated HIV gp140 envelope protein and a fluorescent internalisation probe, opsonised with Abs and incubated with a monocytic cell line. The fluorescence derived from the fluorescent internalisation probe on surface-bound beads, but n..

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