HIV-specific antibody-dependent phagocytosis matures during HIV infection
Fernanda Ana-Sosa-Batiz, Angus PR Johnston, Haiyin Liu, Robert J Center, Supachai Rerks-Ngarm, Punnee Pitisuttithum, Sorachai Nitayaphan, Jaranit Kaewkungwal, Jerome H Kim, Nelson L Michael, Anthony D Kelleher, Ivan Stratov, Stephen J Kent, Marit Kramski
IMMUNOLOGY AND CELL BIOLOGY | WILEY | Published : 2014
Antibody-dependent phagocytosis (ADP) is a potentially important immune mechanism to clear HIV. How HIV-specific ADP responses mature during HIV infection or in response to vaccinations administered, including the partially successful RV144 HIV vaccine, is not known. We established a modified ADP assay to measure internalisation of HIV antibody (Ab)-opsonised targets using a specific hybridisation internalisation probe. Labelled beads were coated with both biotinylated HIV gp140 envelope protein and a fluorescent internalisation probe, opsonised with Abs and incubated with a monocytic cell line. The fluorescence derived from the fluorescent internalisation probe on surface-bound beads, but n..View full abstract
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Awarded by NHMRC
This work was supported by NHMRC program grant no. 510448. The views expressed in this manuscript are those of the authors and do not reflect the views of the US Army or the US Department of Defense. We thank the RV144 trial participants, the whole RV144 study team and all patients and blood donors. We thank Dr Wendy Winnall for statistical advice.