Journal article
Dual DNA binding and coactivator functions of Aspergillus nidulansTamA, a Zn(II)2Cys6 transcription factor
DJ Downes, MA Davis, KH Wong, SD Kreutzberger, MJ Hynes, RB Todd
Molecular Microbiology | WILEY | Published : 2014
DOI: 10.1111/mmi.12620
Abstract
Transcription factors containing DNA binding domains generally regulate transcription by direct interaction with DNA. For most transcription factors, including the fungal Zn(II)2Cys6 zinc binuclear cluster transcription factors, the DNA binding motif is essential for function. However, Aspergillus nidulansTamA and the related Saccharomyces cerevisiaeDal81p protein contain Zn(II)2Cys6 motifs shown to be dispensable for function. TamA acts at several promoters as a coactivator of the global nitrogen GATA transcription factor AreA. We now show that TamA is the major transcriptional activator of gdhA, encoding the key nitrogen metabolism enzyme NADP-glutamate dehydrogenase. Moreover, activation ..
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Awarded by Australian Research Council
Funding Acknowledgements
We thank A. Andrianopoulos for assistance with bioinformatics, G.Y. Busot for assistance with protein manipulation, and acknowledge expert technical assistance of C. C. Hunter, and B. T. Pfannenstiel. This work was supported by the Australian Research Council's Discovery Project funding scheme Project Number DP0558002, Kansas NSF EPSCoR First Award EPS-0903806, The Kansas State University Plant Biotechnology Center, and the Johnson Center for Basic Cancer Research. Contribution No. 14-065-J from the Kansas Agricultural Experiment Station. The authors have no conflict of interest to declare.