Journal article
Stability of gene expression and epigenetic profiles highlights the utility of patient-derived paediatric acute lymphoblastic leukaemia xenografts for investigating molecular mechanisms of drug resistance
NC Wong, VA Bhadri, J Maksimovic, M Parkinson-Bates, J Ng, JM Craig, R Saffery, RB Lock
BMC Genomics | Published : 2014
Abstract
Background: Patient-derived tumour xenografts are an attractive model for preclinical testing of anti-cancer drugs. Insights into tumour biology and biomarkers predictive of responses to chemotherapeutic drugs can also be gained from investigating xenograft models. As a first step towards examining the equivalence of epigenetic profiles between xenografts and primary tumours in paediatric leukaemia, we performed genome-scale DNA methylation and gene expression profiling on a panel of 10 paediatric B-cell precursor acute lymphoblastic leukaemia (BCP-ALL) tumours that were stratified by prednisolone response.Results: We found high correlations in DNA methylation and gene expression profiles be..
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Funding Acknowledgements
This work was supported by an NHMRC Project Grant to JC, RS and RBL, and an NHMRC Research Fellowship to RBL. NW has been supported by the Leukaemia Foundation of Australia, My Room and the Children's Cancer Centre Foundation. VB was supported by a scholarship from the Leukaemia Foundation of Australia. The Murdoch Childrens Research Institute is supported by the Victorian Operational Infrastructure Grant. We thank Dr Benjamin Ong for assistance with the SEQUENOM Facility. Children's Cancer Institute Australia for Medical Research is affiliated with the University of New South Wales and the Sydney Children's Hospitals Network.