Journal article
Plant cytokinesis is orchestrated by the sequential action of the TRAPPII and exocyst tethering complexes
K Rybak, A Steiner, L Synek, S Klaeger, I Kulich, E Facher, G Wanner, B Kuster, V Zarsky, S Persson, FF Assaad
Developmental Cell | Published : 2014
Abstract
Plant cytokinesis is initiated in a transient membrane compartment, the cell plate, and completed by a process of maturation during which the cell plate becomes a cross wall. How the transition from juvenile to adult stages occurs is poorly understood. In this study, we monitor the Arabidopsis transport protein particle II (TRAPPII) and exocyst tethering complexes throughout cytokinesis. We show that their appearance is predominantly sequential, with brief overlap at the onset and end of cytokinesis. The TRAPPII complex is required for cell plate biogenesis, and the exocyst is required for cell plate maturation. The TRAPPII complex sorts plasma membrane proteins, including exocyst subunits, ..
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Awarded by Max-Planck-Gesellschaft
Funding Acknowledgements
We thank Prof. Grill and members of the Botany Department for support. Silvia Dobler helped with sample preparation. Matyas Fendrych prepared the EXO84b antibody. Alex Ivakov helped with spinning disk confocal microscopy. Ngoc Nguyen provided technical assistance. We thank TAIR and NASC for insertion lines. Thanks to Heather McFarlane for useful suggestions and a critical evaluation of the manuscript. We are grateful to Eva Benkova and Christian Luschnig for sharing published resources. Distribution of the JIM7 antibody was supported in part by NSF grant DBI-0421683 and RCN 009281. K.R. and A.S. were supported by DFG grants AS110/4-6 and AS110/5-1; L.S. and V.Z. by Czech Science Foundation grants GPP501/11/P853 and GACR P305/11/1629, respectively; and I.K. by a Charles University grant (Prague project 658112). S.P. was funded by the Max Planck Gesellschaft. This research was funded by DFG grants to F.F.A. and G.W.