Journal article
Culture environment regulates amino acid turnover and glucose utilisation in human ES cells
J Rathjen, C Yeo, C Yap, BSN Tan, PD Rathjen, DK Gardner
Reproduction Fertility and Development | CSIRO PUBLISHING | Published : 2014
DOI: 10.1071/RD12276
Abstract
Human embryonic stem (ES) cells have been proposed as a renewable source of pluripotent cells that can be differentiated into various cell types for use in research, drug discovery and in the emerging area of regenerative medicine. Exploitation of this potential will require the development of ES cell culture conditions that promote pluripotency and a normal cell metabolism, and quality control parameters that measure these outcomes. There is, however, relatively little known about the metabolism of pluripotent cells or the impact of culture environment and differentiation on their metabolic pathways. The effect of two commonly used medium supplements and cell differentiation on metabolic in..
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Grants
Awarded by Molecular Biometrics Pty. Ltd. (New Haven, CT, USA)
Awarded by Australian Research Council
Funding Acknowledgements
The authors would like to thank Dr. Alex Harvey and Dr. John Sheedy (both University of Melbourne) for assistance with the data analysis, Ms. Natasha Dodge, Ms. Mai Truang and Ms. Jackie Yu for technical assistance and Dr. Jeff Mann for the ES cell-tested serum. This work was supported by a Linkage grant from the Australian Research Council and Molecular Biometrics Pty. Ltd. (New Haven, CT, USA) (Grant #LP0989598) and by Stem Cells Australia. C. Yap was supported by an Australian Postgraduate Award and the Australian Stem Cell Centre. B. S. N. T. was supported by a postgraduate scholarship with the University of Melbourne.