Impacts of the Callipyge Mutation on Ovine Plasma Metabolites and Muscle Fibre Type
Juan Li, Paul L Greenwood, Noelle E Cockett, Tracy S Hadfield, Tony Vuocolo, Keren Byrne, Jason D White, Ross L Tellam, Horst Joachim Schirra
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2014
The ovine Callipyge mutation causes postnatal muscle hypertrophy localized to the pelvic limbs and torso, as well as body leanness. The mechanism underpinning enhanced muscle mass is unclear, as is the systemic impact of the mutation. Using muscle fibre typing immunohistochemistry, we confirmed muscle specific effects and demonstrated that affected muscles had greater prevalence and hypertrophy of type 2X fast twitch glycolytic fibres and decreased representation of types 1, 2C, 2A and/or 2AX fibres. To investigate potential systemic effects of the mutation, proton NMR spectra of plasma taken from lambs at 8 and 12 weeks of age were measured. Multivariate statistical analysis of plasma metab..View full abstract
The authors acknowledge financial support provided by the Queensland State Government to the Queensland NMR Network facilities, as well as project and fellowship support (to Juan Li) from the CSIRO Office of the Chief Scientist. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.