Journal article

An Evaluation of Dose Equivalence between Synchrotron Microbeam Radiation Therapy and Conventional Broadbeam Radiation Using Clonogenic and Cell Impedance Assays

Mohammad Johari Ibahim, Jeffrey C Crosbie, Yuqing Yang, Marina Zaitseva, Andrew W Stevenson, Peter AW Rogers, Premila Paiva

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2014

Abstract

BACKGROUND: High-dose synchrotron microbeam radiation therapy (MRT) has shown the potential to deliver improved outcomes over conventional broadbeam (BB) radiation therapy. To implement synchrotron MRT clinically for cancer treatment, it is necessary to undertake dose equivalence studies to identify MRT doses that give similar outcomes to BB treatments. AIM: To develop an in vitro approach to determine biological dose equivalence between MRT and BB using two different cell-based assays. METHODS: The acute response of tumour and normal cell lines (EMT6.5, 4T1.2, NMuMG, EMT6.5ch, 4T1ch5, SaOS-2) to MRT (50-560 Gy) and BB (1.5-10 Gy) irradiation was investigated using clonogenic and real time c..

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Grants

Awarded by National Health and Medical Research Council (NHMRC) (Australia) Project Grant


Awarded by Cancer Council Victoria


Awarded by NHMRC Postdoctoral Early Career Fellowship


Funding Acknowledgements

The work was supported by National Health and Medical Research Council (NHMRC) (Australia) Project Grant (#606614) to PAR and by Cancer Council Victoria (#1048205). PP and JCC were supported by a NHMRC Postdoctoral Early Career Fellowship (#1013774 and #1036174). MJI was sponsored by the Malaysian Government under University Staff Academic Scheme(SLAI). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.