Journal article
Genomic and biochemical analysis of the diaminopimelate and lysine biosynthesis pathway in Verrucomicrobium spinosum: Identification and partial characterization of L,L-diaminopimelate aminotransferase and UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-meso-diaminopimelate ligase
VR Nachar, FC Savka, SE McGroty, KA Donovan, RA North, RCJ Dobson, LJ Buckley, AO Hudson
Frontiers in Microbiology | Published : 2012
Abstract
The Gram-negative bacterium Verrucomicrobium spinosum has attracted interest in recent years following the sequencing and annotation of its genome. Comparative genomic analysis of V. spinosum using diaminopimelate/lysine metabolic genes from Chlamydia trachomatis suggests that V. spinosum employs the L,L-diaminopimelate aminotransferase (DapL) pathway for diaminopimelate/lysine biosynthesis. The open reading frame corresponding to the putative dapL ortholog was cloned and the recombinant enzyme was shown to possess L,L-diaminopimelate aminotransferase activity in vitro. In vivo analysis using functional complementation confirmed that the dapL ortholog was able to functionally complement an E..
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Awarded by National Science Foundation
Funding Acknowledgements
This research was supported by a United States National Science Foundation (NSF) award to AOH (MCB-1120541). Victoria R. Nachar was supported by an Undergraduate Summer Research Fellowship by the College of Science at RIT Victoria R. Nachar and Francisco C. Savka are enrolled in the Biology Program and SEM is enrolled in Bioinformatics Program at RIT. Victoria R. Nachar, Francisco C. Savka, and Sean E. McGroty was supported by the NSF award (MCB-1120541) to Andre O. Hudson, Renwick C. J. Dobson acknowledges the CR Roper Bequest for Fellowship support, the New Zealand Royal Society Marsden Fund (UOC1013) for funding support and that this material is based upon work supported in part by the U. S. Army Research Laboratory and the U. S. Army Research Office under contract/grant number W911NF-11-1-0481. Katherine A. Donovan and Rachel A. North acknowledge the University of Canterbury Summer Scholarship Scheme, Andre O. Hudson thanks Naomi L. Ward form the University of Wyoming for graciously providing the V. spinosum strain/genomic DNA. The authors thank Michael A. Savka (RIT) for helpful discussions and revisions of the manuscript.