Comparison of functional profiles at human recombinant somatostatin sst 2 receptor: Simultaneous determination of intracellular Ca 2 and luciferase expression in CHO-K1 cells

Abstract

1. Somatostatin (somatotropin release inhibiting factor; SRIF) acts via five G protein-coupled receptors (sst 1-sst 5) that modulate multiple cellular effectors. The aim of this study was to compare two functional effects of the human sst 2 receptor stably expressed in CHO-K1 cells in a single experiment using a duplex assay for intracellular calcium and serum response element (SRE)-driven luciferase expression. 2. Intracellular calcium was measured using a fluorometric imaging plate reader II (FLIPR II). SRIF-14 rapidly and transiently increased intracellular calcium with a pEC 50 of 8.74 ± 0.03 (n = 52). At 5 h after FLIPR II measurements, luciferase expression was determined. SRIF-14 conc..