Measuring Turnover of SIV DNA in Resting CD4 T Cells Using Pyrosequencing: Implications for the Timing of HIV Eradication Therapies
Jeanette C Reece, Alexey Martyushev, Janka Petravic, Andrew Grimm, Shayarana Gooneratne, Thakshila Amaresena, Robert De Rose, Liyen Loh, Miles P Davenport, Stephen J Kent
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2014
Resting CD4+ T cells are a reservoir of latent HIV-1. Understanding the turnover of HIV DNA in these cells has implications for the development of eradication strategies. Most studies of viral latency focus on viral persistence under antiretroviral therapy (ART). We studied the turnover of SIV DNA resting CD4+ T cells during active infection in a cohort of 20 SIV-infected pigtail macaques. We compared SIV sequences at two Mane-A1*084:01-restricted CTL epitopes using serial plasma RNA and resting CD4+ T cell DNA samples by pyrosequencing, and used a mathematical modeling approach to estimate SIV DNA turnover. We found SIV DNA turnover in resting CD4+ T cells was slow in animals with low chron..View full abstract
Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
Supported by the Australian National Health and Medical Research Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.