Human mesenchymal stem cells alter macrophage phenotype and promote regeneration via homing to the kidney following ischemia-reperfusion injury
Andrea F Wise, Timothy M Williams, Mensiena BG Kiewiet, Natalie L Payne, Christopher Siatskas, Chrishan S Samuel, Sharon D Ricardo
American Journal of Physiology. Renal physiology | AMER PHYSIOLOGICAL SOC | Published : 2014
Mesenchymal stem cells (MSCs) ameliorate injury and accelerate repair in many organs, including the kidney, although the reparative mechanisms and interaction with macrophages have not been elucidated. This study investigated the reparative potential of human bone marrow-derived MSCs and traced their homing patterns following administration to mice with ischemia-reperfusion (IR) injury using whole body bioluminescence imaging. The effect of MSCs on macrophage phenotype following direct and indirect coculture was assessed using qPCR. Human cytokine production was measured using multiplex arrays. After IR, MSCs homed to injured kidneys where they afforded protection indicated by decreased prox..View full abstract
Awarded by National Health and Medical Research Council (NHMRC) of Australia
This study was funded by a Project Grant (1003806) from the National Health and Medical Research Council (NHMRC) of Australia. C. S. Samuel is supported by Monash University Mid-Career and NHMRC Senior Research Fellowships.