Conference Proceedings

Roles of the Receptor, the Ligand, and the Cell in the Signal Transduction Pathways Utilized by the Relaxin Family Peptide Receptors 1-3

Roger J Summers, Ross AD Bathgate, John D Wade, Emma T van der Westhuizen, Michelle L Halls, GD BryantGreenwood (ed.), CA Bagnell (ed.), RAD Bathgate (ed.)

Annals of the New York Academy of Sciences | BLACKWELL PUBLISHING | Published : 2009

Abstract

The relaxin-like peptides produce their effects by acting at four G-protein-coupled receptors (GPCRs) RXFP1 to 4. RXFP1 and 2 are characterized by large extracellular domains containing leucine-rich repeats, whereas RXFP3 and 4 closely resemble small-peptide-liganded GPCRs. Studies with mutant RXFP1 receptors established that the final 10 amino acids of the C-terminus and Arg(752) in particular are obligatory for the second phase of cAMP signaling. Examination of the importance of cell type revealed different patterns of cAMP signaling related to the types of G-proteins expressed in these cells. Studies of RXFP3 signaling using reporter genes revealed that both relaxin and relaxin-3 activate..

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Funding Acknowledgements

We would like to acknowledge Corthera Inc. for the recombinant relaxin used in these studies. Emma T. van der, Westhuizen is supported by an NH&MRC Australia Dora Lush and Monash University departmental postgraduate scholarship and a Relaxin 2008 Young Investigator Award. Michelle L. Halls is the recipient of an NH&MRC Overseas Biomedical Fellowship and a Relaxin 2008 Young Investigator Award. This research was supported by NH&MRC Australia project grants 436713 (RJS and RADB) and ARC linkage grant LP0560620 (RJS and RADB).