Journal article

Activation of Relaxin-Related Receptors by Short, Linear Peptides Derived from a Collagen-Containing Precursor

Ronen Shemesh, Chen Hermesh, Amir Toporik, Zurit Levine, Amit Novik, Assaf Wool, Yossef Kliger, Avi Rosenberg, Ross AD Bathgate, Yossi Cohen, GD BryantGreenwood (ed.), CA Bagnell (ed.), RAD Bathgate (ed.)

Annals of the New York Academy of Sciences | WILEY-BLACKWELL | Published : 2009


In a screening effort based on algorithmic predictions for novel G-protein-coupled receptor (GPCR) peptide activators, we were able to identify and examine two novel peptides (P59 and P74) which are short, linear, and derived from a natural, previously unidentified precursor protein containing a collagen-like repeat. Both peptides seemed to show an apparent cAMP-related effect on CHO-K1 cells transiently transfected with either LGR7 or LGR8, usually after treatment with cAMP-generating forskolin, compared to the same cells treated with forskolin plus relaxin. This activation was not found for the relaxin-3 receptor (GPR135). In a set of follow-up experiments, both peptides were found to stim..

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