Identification of the MAT1 locus in Stagonosporopsis tanaceti, and exploring its potential for sexual reproduction in Australian pyrethrum fields
Niloofar Vaghefi, Peter K Ades, Frank S Hay, Sarah J Pethybridge, R Ford, Paul WJ Taylor
Fungal Biology | ELSEVIER SCI LTD | Published : 2015
Stagonosporopsis chrysanthemi, S. inoxydabilis, and S. tanaceti are closely related Ascomycetes associated with ray blight of the Asteraceae. To date, only S. tanaceti has been identified in Australia, incurring substantial losses to the pyrethrum industry. In contrast to the homothallic S. chrysanthemi and S. inoxydabilis, a sexual state has not been observed for S. tanaceti. The MAT1 locus in S. tanaceti was identified through de novo assembly of shotgun reads, and was further used to develop primers for amplification of the full-length MAT1/2 locus in S. chrysanthemi and S. inoxydabilis. As expected, S. chrysanthemi and S. inoxydabilis possessed a MAT1/2 locus typical of homothallic Dothi..View full abstract
We would like to thank Ms Stacey Pilkington and Pattie Weichelt for technical support. This project was supported by Botanical Resources Australia - Agricultural Services Pty. Ltd.