Journal article
Genes Found Essential in Other Mycoplasmas Are Dispensable in Mycoplasma bovis
Shukriti Sharma, Philip F Markham, Glenn F Browning
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2014
Abstract
Mycoplasmas are regarded to be useful models for studying the minimum genetic complement required for independent survival of an organism. Mycoplasma bovis is a globally distributed pathogen causing pneumonia, mastitis, arthritis, otitis media and reproductive tract disease, and genome sequences of three strains, the type strain PG45 and two strains isolated in China, have been published. In this study, several Tn4001 based transposon constructs were generated and used to create a M. bovis PG45 insertional mutant library. Direct genome sequencing of 319 independent insertions detected disruptions in 129 genes in M. bovis, 48 of which had homologues in Mycoplasma mycoides subspecies mycoides ..
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Funding Acknowledgements
These studies were partially supported by financial support from the Australian Government under the AusAID program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No additional external funding was received for this study.