Journal article

Peptide self-association in aqueous trifluoroethanol monitored by pulsed field gradient NMR diffusion measurements

SG Yao, GJ Howlett, RS Norton

Journal of Biomolecular NMR | SPRINGER | Published : 2000


Defining the self-association state of a molecule in solution can be an important step in NMR-based structure determination. This is particularly true of peptides, where there can be a relatively small number of long-range interactions and misinterpretation of an intermolecular NOE as an intramolecular contact can have a dramatic influence on the final calculated structure. In this paper, we have investigated the use of translational self-diffusion coefficient measurements to detect self-association in aqueous trifluoroethanol of three peptides which are analogues of the C-terminal region of human neuropeptide Y. Experimentally measured diffusion coefficients were extrapolated to D0, the lim..

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University of Melbourne Researchers