Journal article

Hypoxic preconditioning produces differential expression of hypoxia-inducible factor-1 alpha (HIF-1 alpha) and its regulatory enzyme HIF prolyl hydroxylase 2 in neonatal rat brain

Nicole M Jones, Elizabeth M Lee, Timothy G Brown, Bevyn Jarrott, Philip M Beart

NEUROSCIENCE LETTERS | ELSEVIER IRELAND LTD | Published : 2006

Abstract

Hypoxic preconditioning can protect the brain against a subsequent damaging ischaemic insult. Mild hypoxia alone seems not sufficient to cause neuronal injury, but can induce changes in gene expression and intracellular signalling pathways and the hypoxia-inducible transcription factor (HIF-1) is a key modulator of these genes. Recently, a family of HIF prolyl hydroxylase enzymes (PHDs) has been shown to regulate HIF-1 function by controlling its degradation. Since PHD-2 is thought to be the predominant isoform which regulates HIF-1, we have investigated whether preconditioning with hypoxia can affect levels of PHD-2 and HIF-1alpha proteins to elucidate roles for the HIF-1/PHD-2 system in th..

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